How to decalcify tissues for Histopathological processing

Bone for Decalcification

The presence of calcium salts in tissues makes them difficult. This causes knife damage, tissue cutting difficulty. Calcium may also be present in normal tissues under pathological conditions such as necrotic tissue in tuberculosis. Decalcification is the process of removing calcium salts Why is Decalcificaition…

Papanicolaou stains : Principle ,Procedure and Interpretation

Papanicolaou stain : Principle ,Procedure and Result Interpretation

Papanicolaou stains are used to differentiate cells in smear preparations of various body secretions. Pap stain is a very reliable technique. As such, it is used for cervical cancer screening.Malignant cells are generally characterized by relatively large nuclei with an abnormal chromatin pattern and small…