Auramine Phenol staining technique for Acid Fast Bacilli

Auramine Phenol stain Mycobacterium tuberculosis MLTGEEKS

Phenolic Auramine is used as primary staining solution in fluorescent staining. It is a staining technique mostly used because of it high sensitivity to detect mycobacterium tuberculosis in sputum,csf and other specimen as compared to ziehl neelsen. It is a fluorochrome technique which enables a…

Acid Fast Bacilli Ziehl Neelsen staining technique Principle, Procedure and reporting

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Acid-fastness  refers to  a physical property of certain bacterial or group of bacteria and eukaryotic cells, as well as some sub-cellular structures found in a organism, which makes it resistant to decolorization by the acid alcohol during laboratory staining procedures Acid-fast organisms are characterized by…

Acid Fast organisms not Always Mycobacterium species

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Nowaday,when we hear of acid fast organism,we primarily think of Mycobacterium species such as tuberculosis,leprae etc.           Acid-fast organisms such as members of the genus Mycobacterium are  differentiated and identify using an acid fast stain such as Ziehl Neelsen stain Acid-fastness  refers…