Culture methods in Microbiology

In Microbiology laboratory, Culture methods are very crucial as it increases the chances of multiplication and identification of microorganism. The following are various reasons for culturing microorganisms in the laboratory: Isolate bacteria in pure culture and identify the same by performing various tests. In order…

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas is a bacteria mostly saprophytic in nature, found in soil, water and other moist environments. It has emerged as an important cause of associated health care and opportunistic infections.Most Pseudomonas clinical isolates are resistant to many antibiotics. Pseudomonas aeruginosa is also a plant pathogen….

Kligler Iron Agar principles,composition and slants interpretation

Kligler iron slant MLTGEEKS

Kligler Iron Agar abbreviated KIA is a growth media recommended for the differential identification of gram-negative enteric bacilli from clinical and non clinical samples based on the fermentation  sugars that is dextrose, lactose and the production of Hydrogen sulphide Those bacteria that ferment any of…

Brain infusion Broth and Agar Principle and composition and growth response

Brain heart infusion agar MLTGEEKS

Brain heart infusion (BHI) is a nutritious growth medium used to culture a variety of fastidious organisms associated with blood culture work and allied pathological investigations. This nutritious medium has been used to culture pathogenic cocci such as streptococci, pneumococci and meningococci  which are can…

Egg Yolk Agar Base Selective and Differential Medium for the Isolation of Clostridium perfringens

Egg Yolk base agar MLTGEEKS

There are some bacteria (Clostridium perfringens) can produce enzymes (lipases) that hydrolyze lipids like fats, steroids,oils, lecithins, glycerol etc especially in food. The enzyme lipases is used by these bacteria for the hydrolysis of phospholipid in cell membranes, allowing skin and subcutaneous tissues colonization.This is…

tryptone soya agar (soybean casein digest agar) Principles,composition and growth characteristics

staphylococcus aureus on TSA

Soyabean Casein Digest Agar also know as Tryptone Soya Agar or broth  (TSA) is a general purpose growth media used for the culture of a wide variety of microorganisms including fastidious organism like Neisseria species,Brucella,Haemophilus species and Listeria etc . With an addition of blood,this…

Triple Sugar Iron Agar Principles,composition and colony characteristics

triple-sugar-iron-agar-test

Triple Sugar Iron Agar  was originally proposed by Sulkin and Willett and modified by Hajna for the identification of  Enterobacteriaceae.It is selective growth media recommended for identification of gram-negative enteric bacilli on the basis of  sugars such as dextrose, lactose and sucrose fermentation and hydrogen…

Simmons’ citrate agar Principles,composition and colony characteristics

Simmon-citrate-agar-A-Green-No-growth-B-GreenPositive-Growth

Simmons’ citrate agar is differential growth media recommended for  gram-negative bacteria or members Enterobacteriaceae  on the basis of citrate utilization.That is,useful for organisms that uses citrate as a carbon and source of energy. It is also defined as  a selective and differential medium that tests…

Selenite Fluid Broth Principles,composition and colony characteristics

culture-media-prac-microbiology-24-638

originated by Leifson,Selenite  Broth is recommended as an enrichment medium for the isolation of Salmonella species from clinical and non clinical specimens such as faeces, urine or other pathological materials Principle of Selenite Fluid Broth The peptone present in Selenite broth provides nitrogenous substances Lactose…

Müller-Hinton agar principle and antimicrobial susceptibility testing

Mueller-Hinton-agar-media-with-antibiotic-sensitivity-disc-showing-Oxacillin-sensitive

Müller-Hinton agar formulation was originally developed as a simple, transparent agar medium for the growth of pathogenic Neisseria species by John Howard Mueller and Jane Hinton at Harvard.It is now used as a test medium for antimicrobial susceptibility testing .It is recommended for the diffusion…