Dnase differential test Principle,Procedure and Result

dnase test MLTGEEKS

Deoxyribonuclease abbreviated DNase  is an enzyme that catalyzes  the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thereby degrading DNA. Some microorganisms like Staphylococcus species possesses this enzymes hence Dnase test agar is a differential medium that test the ability of Dnase producing microorganisms…

ONPG test principles,Composition and Cultural response

ONPG TEST MLTGEEKS

Ortho-nitrophenyl-b-D-galactopyranoside abbreviated ONPG is a microbiological biochemical test used for the rapid detection of  the enzyme b-galactosidase activity in some microorganisms, especially those late lactose fermenters (cryptic lactose fermenters). Lactose fermentation by microorganisms requires a couple of enzymes and one of which is beta-galactosidase ONPG…

Bile solubility test for Streptococcus pneumoniae

Bile-Solubility-Test

The bile solubility testing is microbiology biochemical test that helps to differentiate bile and bile salts soluble organism like Streptococcus pneumoniae from other insoluble bile and bile salt alpha haemolytic  streptococci such as streptococcus viridans. Principle of Bile solubility test Based on the emulsification of…

Biochemical catalase test principle and interpretations (All catalase positive organism)

Catalase Test Staph Strep

The Catalase biochemical test is used to differentiate those bacteria that are able to produce the enzyme catalase from non catalase producing bacteria.This test is mostly used for the differentiation of Catalase postive staphylococci from catalase negative streptococci  Principle of Catalase test There are two…

Bacteria Oxidase Biochemical test (Fresh and Test Strip method)

Oxidase test

The oxidase biochemical test was originally devised to identify Neisseria species, but later was used to separate the Pseudomonadaceae from the oxidase-negative Enterobacteriaceae.This test identifies organisms that produce the enzyme cytochrome oxidase that participates in the electron transport chain by transferring electrons from a donor…