Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium (Oxidative-Fermentative Medium ) is used to distinguish between anaerobic and aerobic breakdown of carbohydrate( glucose).Hugh Leifson Medium was developed by Hugh and Leifson.They described the taxonomic significance of fermentation and oxidative metabolism of carbohydrates in gram- negative intestinal bacteria.There are two ways…

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

The Proteus and Providencia genera belong to the Proteeae of the Enterobacteriaceae family. The members of both these genera are gram negative, motile bacilli, aerobic and facultative anaerobic and can grow in basic media.A characteristic feature that distinguishes tribe Proteeae from other members of Enterobacteriaceae…

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas is a bacteria mostly saprophytic in nature, found in soil, water and other moist environments. It has emerged as an important cause of associated health care and opportunistic infections.Most Pseudomonas clinical isolates are resistant to many antibiotics. Pseudomonas aeruginosa is also a plant pathogen….

Thioglycollate Broth principles and Bacteria pattern of Growth

Fluid Thioglycollate medium mltgeeks

Thioglycollate Broth or Fluid Thioglycollate medium is used for sterility testing of antibiotics, biologicals specimen such as stored blood in blood banks,foods and for determination of the phenol coefficient and sporicidal effect of disinfectants.It is also used for the culture and isolation of obligate,facultative anaerobic…