Schaeffer & Fulton’s Spore Stain principle,procedure and result (endospore stain)

Schaeffer & Fulton's Spore Stain MLTGEEKS

Schaeffer & Fulton’s Spore Stain is a microbiological stain used for staining bacterial spores.A spore is a dormant form of a bacterium that allows it to survive in drastic environmental conditions. Spores have a tough external cover made of protein keratin that is resistant to heat and chemicals. Keratin also resists staining, so extreme measures are needed to stain the spore.

Principle of Schaeffer & Fulton’s Spore Stain

  • A primary stain- malachite green is forced into the spore by steaming the bacterial emulsion in the Schaeffer- Fulton method.Malachite green is soluble in water and has low affinity for cellular material, so vegetative cells can be discolored with water.
  • Vegetative cells are then countered by safranin
  • Spores may be located in the center of the cell, at the end or between the end and middle of the cell.
  • Spore shape may also be used for diagnostic purposes. Spores might be spherical or elliptic.
  • Corynebacterium members may exhibit club- shaped swellings that may be confused with spores. Spore staining differs between real spores and those structures.

Composition of Schaeffer & Fulton’s Spore Stain

Schaeffer & Fulton’s Spore Stain A

Malachite green5.000 gram
Distilled water100.000 ml

Schaeffer & Fulton’s Spore Stain B

Safranin O0.500 gram
Distilled water100.000 ml

Procedure for spore staining using  Schaeffer & Fulton’s Spore Stain

Schaeffer & Fulton's Spore Stain procedure
Schaeffer & Fulton’s Spore Stain procedure
  1. Prepare a smear of the culture. Air dry it and fix with gentle heat.
  2. Flood the entire slide, with aqueous malachite green/Schaeffer and Fulton Spore Stain A
  3. Steam for 3-6 minutes, and rinse under running tap water.
  4. Counterstain with 0.5% aqueous safranin/Schaeffer and Fulton Spore Stain B for 30 seconds.
  5. Wash with water, dry and observe under oil immersion lens.

Observation and Interpretation

Spore staining is performed and the characteristics of the organism are observed under the microscope with the use of the oil immersion lens.

Also read  How to prepare tryptic Soy Agar(TSA) : Principle,Composition and Colony response

Sporesgreen
Vegetative cells Red
Schaeffer & Fulton's Spore Stain

Schaeffer & Fulton’s Spore Stain 

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About the Author: Arthur Westmann

DEFFE ARTHUR (AMOEBAMANN) is the founder and author of MLTGEEKS and MLTEXPO.He’s from Cameroon and is currently a Final year State Medical Laboratory Technician (MLT MA). Beyond lab works, he’s a passionate internet user with a keen interest in web design and blogging. Furthermore He likes traveling, hanging around with friends and social networking to do in his spare time.

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