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Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium (Oxidative-Fermentative Medium ) is used to distinguish between anaerobic and aerobic breakdown of carbohydrate( glucose).Hugh Leifson Medium was developed by Hugh and Leifson.They described the taxonomic significance of fermentation and oxidative metabolism of carbohydrates in…

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

The Proteus and Providencia genera belong to the Proteeae of the Enterobacteriaceae family. The members of both these genera are gram negative, motile bacilli, aerobic and facultative anaerobic and can grow in basic media.A characteristic feature that distinguishes…

Overview of Bacteria identification test procedures and Interpretations

Overview of Bacteria identification test procedures and Interpretations

Isolated bacteria must be further identified at genus and species level. The identification of bacteria is required to cure the disease or infection caused by the bacteria by using appropriate antibiotics. Identification is also important for epidemiological purposes….

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas is a bacteria mostly saprophytic in nature, found in soil, water and other moist environments. It has emerged as an important cause of associated health care and opportunistic infections.Most Pseudomonas clinical isolates are resistant to many antibiotics….

Brugia malayi Laboratory Diagnosis

Brugia malayi Laboratory diagnosis and Treatment

Areas of the world in which mosquitoes breed are the primary places in which Brugia malayi can be found.These include the Philippines, Indonesia, Sri Lanka, New Guinea, Vietnam, Thailand and Japan, Korea and China.Although humans are considered the…

museum fixative and preservation

How to preserve museum specimens (Museum mounting techniques)

All teaching hospitals and colleges of pathology have museums that serve many functions: permanent exhibition of common specimens for undergraduate and postgraduate teaching purposes such as collected animals, illustration of rare specimens, permanent source of histological material and…

Chlamydia trachomatis Laboratory Diagnosis

Chlamydia trachomatis Laboratory Diagnosis

Chlamydiae are obligatory, aerobic, intracellular parasites of eukaryotic cells.Chlamydiae are obligatory, aerobic, intracellular parasites of eukaryotic cells.They are small gram- negative cocci or rod- shaped, non- motile bacteria.Chlamydiae exhibits intermediate characteristics between bacteria and viruses.They are widespread in…

Acanthamoeba infection laboratory diagnosis

Acanthamoeba infection laboratory diagnosis

Over the years, Acanthamoeba cases have been reported from many countries worldwide. In the United States, both CNS and Acanthamoeba eye infections have been reported. CNS infections occur primarily in patients who are immunocompromised or weakened.Contact lenses wearers,…

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium is used in the isolation and cultivation of Mycobacterium species.Solid media used for the isolation and cultivation of Mycobacteria are egg- based or agar- based.Egg- based media contain whole eggs or egg yolk, potato flour,…

WBC (White Blood Cell) Count: Purpose, Procedure, and Results

WBC (White Blood Cell) Count: Principle, Procedure, and Calculation

WBC diluting fluid is used to perform the WBC(leucocyte) count or white blood cell count Principle of W.B.C. Diluting Fluid WBC diluting fluid is used to perform the WBC( leucocyte) count. Red cells are lysed by glacial acetic…