MacConkey Agar Principles, Composition, Preparation, colony characteristics

MacConkey Agar principle and interpretation MLTGEEKS

The culture media MacConkey agar was developed in 20th century by Alfred Theodore MacConkey. MacConkey agar was the first formulated solid differential media but now known as   a selective and differential culture media commonly used for the isolation of enteric Gram-negative bacteria.Based on the…

Venipuncture in Medical Laboratory – Arteries or veins

venipuncture MLT GEEKS

According to Wikipedia, venepuncture or venipuncture is the puncture of a vein as part of a medical procedure, typically to withdraw a blood sample or for an intravenous injection. In order to carry out this process, it is essential to follow a Standard Operating Procedure…

Gram Staining procedure and Interpretation

Gram-positive-vs-gram-negative-bacteria-MLT GEEKS

Gram Staining This is a technique used for the differentiation of Gram-positive and Gram-negative bacteria and is usually almost  the first test performed for the identification of bacteria. The Gram staining method, was named after the Danish bacteriologist who originally devised it in 1882 (published 1884), Hans Christian Gram…

Action of Antibiotics against Microorganisms

Mechanism-of-Action-of-Bacteristatic-Drugs

The following are how antibiotic act against microorganism either In-vivo or In-vitro Protein synthesis Antibacterial action attacks the 30s portion of the ribosomic which causes a misreading of coding of RNA and as a result causes the manufacture of wrong protein. Gentamycin can completely stop…

ACTION OF COMBINED ANTIBACTERIAL AGENTS

Action of antibiotics MLTGEEKS

In Microbiology,it is very important to understand the actions of various antibacterial agents in order to easily guide the physician when giving Chemotherapeutic treatment especially when carrying on susceptibility testing. Combination of antibiotics are often given In case of undiagnosed infections, when a broad cover…

Sabouraud Dextrose Agar (SDA) Composition, Principle, Uses, Preparation culture and Colony Morphology

Rhodotorula_mucilaginosa_colonies MLTGEEKS

Sabouraud Agar or Sabouraud Dextrose Agar or SDA is a type of agar growth medium containing peptones. SDA  is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia. SDA  was created by Raymond Sabouraud in 1892 reason…

Eosin Methylene Blue (EMB) Agar : Principle purpose and colonies characteristics

Eosin Methylene Blue MLTGEEKS

Eosin Methylene Blue (EMB, also known as “levine emb agar formulation”) is a selective and differential culture medium for gram-negative bacteria. It’s contains dyes that are toxic to gram-positive bacteria making it selective. EMB is a selective and differential medium for coliforms bacteria. It is made up of…

TRICHURIA TRICHIURIS MORPHOLOGY ,SYMPTOMS ,LABORATORY DIAGNOSIS AND TREATMENT

TRICHURIA TRICHIURIS Human are infected chiefly by a species of whipworm called Trichuris trichiuris or Trichocephalus trichiuris which are round worm nematode that causes Trichuriasis ,one of the neglected tropical disease that infects human and animals large intestine. Because of the worm shapes (Look like a whip with wider handles at the posterior…

PINWORM (ENTEROBIUS VERMICULARIS) SIGNS AND SYMPTOMS, DIAGNOSTIC AND TREATMENT

Enterobius vermicularis MLTGEEKS

Enterobius Vermicularis Pinworms are prevalent around the world, although they are not a serious threat to the overall health of individual, they do cause some discomfort through anal irritation and nervousness mostly in small children. The most prevalent type of human worm infection in the…

CHEMOTHERAPEUTIC AGENTS AND THEIR USES IN SUSCEPTIBILITY TEST

Antibiotics may sometimes be called Antibacterial are chemotherapeutic agents that are naturally (Antibiotics) and synthetically (Antibacterial) produced and have lethal effect on microorganisms. In other words, Antibiotics are a type of Antimicrobial agent or drugs used in the treatment and prevention of microorganism infections. Antibiotics…