Stool examination (Wet mount vs Lugol iodine staining) Method

stool_sample MLTGEEKS

Stool is one of the most common sample used in the laboratory to diagnose intestinal parasites,Though some serological methods have been develop,the most commonly performed procedure in parasitology is the ova and parasite (O &P) examination base on stool macroscopic and microscopical observation Gastro-intestinal infestations…

Foaming test to detect bile salts and bile pigments in urine

Bile salt test MLTGEEKS

Bile is a yellow-green fluid that contains water and organic molecules such as cholesterol, bile acids, and bilirubin. In humans, the two main function of bile are the digestion and absorption of fats and the elimination of bile salts from the body by secretion into…

Mannitol Salt Agar (Selective and different culture media) for Halophilic bacteria

Mannitol salt agar MLTGEEKS

Abbreviate MSA, Mannitol salt agar  is a selective and differential culture medium used in microbiology for the growth and isolation of Gram positive Staphylococci species such as Staphylococcus aureus. It was described by Chapman and has been adopted by many official organisations. Several modifications of it have been developed, all formulations…

Thiosulfate Citrate Bile Sucrose Agar ideal for vibrio species

Abbreviated TCBS, Thiosulfate-Citrate-Bile-Sucrose Agar was original developed by Nakanishi but was subsequently modified by Kobayashy et al. TCBS is frequently used for the selective isolation of Vibrio cholerae and other enteropathogenic like Vibrios parahaemolyticus from suspected specimen. TCBS agar is both selective and differential culture…

BACTERIA IDENTIFICATION BY KOH STRING TEST

KOH-Test-for-Gram-stain-confirmation

The Gram staining reaction is not always effective in the indications of the organism’s cell wall structure. Poorly controlled decolorizing, wrong solution dilution and negligence of the technician can obviously be a source of errors resulting during gram staining, but even with adequate technique, some…

Urine Dipstick principle and significance of parameters

Urine test stripes examined by aN MLT

Urine dipstick  are point of care easy to use, cost-effective medical tool that allow for the specific diagnosis and effective treatment of various disease states. The test strips are designed to detect small pathological changes in urine, and provide the result via a color-change test…

Introduction to Histopathology

Introduction to Histopathology MLTGEEKS

What is Histopathology It is a branch of pathology or clinical pathology which deals with  the study of diseases in a tissue section.These tissues undergoes series of steps before reaching the histopathologist desk for microscopic examination. To achieve this,it is always important that the tissue…

CLED Agar Composition, Preparation and Colony growth Characteristics

cled agar MLTGEEKS

Cystine Lactose Electrolyte-Deficient Agar(CLED Agar)  is a type of differential culture media in microbiology recommended for the diagnosis of urinary tract infections. The medium supports the growth of all urinary potential pathogens and provides distinct colony morphology.It also supports the growth of a number of contaminants…

MacConkey Agar Principles, Composition, Preparation, colony characteristics

MacConkey Agar principle and interpretation MLTGEEKS

The culture media MacConkey agar was developed in 20th century by Alfred Theodore MacConkey. MacConkey agar was the first formulated solid differential media but now known as   a selective and differential culture media commonly used for the isolation of enteric Gram-negative bacteria.Based on the…

Venipuncture in Medical Laboratory – Arteries or veins

venipuncture MLT GEEKS

According to Wikipedia, venepuncture or venipuncture is the puncture of a vein as part of a medical procedure, typically to withdraw a blood sample or for an intravenous injection. In order to carry out this process, it is essential to follow a Standard Operating Procedure…