How to decalcify tissues for Histopathological processing

Bone for Decalcification

The presence of calcium salts in tissues makes them difficult. This causes knife damage, tissue cutting difficulty. Calcium may also be present in normal tissues under pathological conditions such as necrotic tissue in tuberculosis. Decalcification is the process of removing calcium salts Why is Decalcificaition…

How to preserve museum specimens (Museum mounting techniques)

museum fixative and preservation

All teaching hospitals and colleges of pathology have museums that serve many functions: permanent exhibition of common specimens for undergraduate and postgraduate teaching purposes such as collected animals, illustration of rare specimens, permanent source of histological material and for gross and microscopic photography. Any museum…

Papanicolaou stains : Principle ,Procedure and Interpretation

Papanicolaou stain : Principle ,Procedure and Result Interpretation

Papanicolaou stains are used to differentiate cells in smear preparations of various body secretions. Pap stain is a very reliable technique. As such, it is used for cervical cancer screening.Malignant cells are generally characterized by relatively large nuclei with an abnormal chromatin pattern and small…

How to decalcify Tissue for Histological Processing

How decalcify Tissue for Histological Processing mltgeeks

The presence of calcium salts in the tissue makes them hard. This causes damage to the knife and difficulty cutting tissue. Calcium is usually found in bones and teeth. Calcium may also be present in normal tissues in pathological conditions, such as necrotic tissue in…

Properties and Principles of Hematoxylin & Eosin staining technique

Hematoxylin and eosin stain mltgeeks

In histopathology,the sections (microtomy) as they are prepared are colourless and different components cannot be appreciated. Staining of tissues(histochemical staining) with different coloured dyeshaving affinities for specific components of the  tissue, makes identification and study of the tissue morphology possible by histotechnologist. There are somany…

Microanatomical,Cytological and Histochemical fixatives

FIXATIVES HISTOPATHOLOGYMLTGEEKS

Some fixatives are made by combining one or more fixative so that the disadvantage of one are reduced by use of another fixative.These types of fixatives are known as compound fixatives.All these compound fixatives have their own advantages and disadvantages and should be used judiciously.Fixatives…

Types and Choice of fixatives used in Histopathology

Fixatives MLTGEEKS

Fixative brings about a crosslinking of protein which denature or coagulate proteins so that the semifluid state is converted into semisolid state so that it maintains everything in vivo in relation to each other.Therefore a semisolid state facilitates easy manipulation of tissues. Choice of Fixative…

Fixation and properties of an ideal fixatives in Histopathology

Fixation in Histopathology MLTGEEKS

Fixation in histopathology is a complex series of chemical events which brings about changes in the various chemical constituents of cell like hardening but doesn’t alter or change the cell structural details and morphology.Instead,fixation is means of preservation of the histopathological specimen. Unless a tissue…

Introduction to Histopathology

Introduction to Histopathology MLTGEEKS

What is Histopathology It is a branch of pathology or clinical pathology which deals with  the study of diseases in a tissue section.These tissues undergoes series of steps before reaching the histopathologist desk for microscopic examination. To achieve this,it is always important that the tissue…