Difference Between Serum And Plasma

Blood plasma

For some scientists and medical students, the difference between serum and plasma is just nomenclature and colour. On more than once occasion we’ve had to ask the difference between Serum and Plasma, only to hear, “either one” or “aren’t they the same thing?”  Let us…

WBC Count : Principle, Procedure and Calculation

WBC (White Blood Cell) Count: Purpose, Procedure, and Results

WBC diluting fluid is used to perform the WBC(leucocyte) count or white blood cell count Principle of WBC Diluting Fluid WBC diluting fluid is used to perform the WBC( leucocyte) count. Red cells are lysed by glacial acetic acid. Gentian violet slightly stains the leukocyte…

Blood Types,Rhesus and ABO Blood Group Test (Beth-Vincent and Simonin blood group test)

lood Types,Rhesus and ABO Blood Group Test MLTGEEKS

Erythrocytes contain a number of antigen types( at least 600) belonging to a membrane grouped in several systems.Since the antibodies formed against antigens (abo antibodies) cause the agglutination of erythrocytes, they are called agglutinogens.The erythrocyte membrane contains A, B and plasma antigens known as agglutinins:…

How to perform Direct and Indirect coombs test

How to perform Direct and Indirect coombs test mltgeeks

The antiglobulin test (Coombs test) was introduced in 1945 by Coombs, Mourant and Race. The test has been developed to detect antibodies that can bind the antigen on the surface of erythrocytes but can not agglutinate them( incomplete antibodies). What is the Principle of Coombs…

R.B.C. Diluting Fluid (Hayem’s) Principle,Procedures and Calculation

R.B.C. Diluting Fluid (Hayem's) MLTGEEKS

It is used as diluent for blood samples to count red blood cells under high power using a manual cell counter or cell counting chamber such as neubauer chamber cell count Principle of  Hayem’s fluid RBC dilution fluid is isotonic with blood, so there is no hemolysis. Normal…

Types and causes of Anemia

Types of Anemia MLTGEEKS

Anemia is a state of reduced red blood cell mass leading to reduced body oxygen carrying capacity.Red cell indexes are useful in the classification of anemia.Anemias are etiologically and morphologically classified.In this article,we will discuss about red cell indices as well as anemia classification.Anemia is…

Various methods used for hemoglobin measurement in the laboratory

Hemoglobin estimation MLTGEEKS

Hemoglobin is the main component of red cell cytoplasm, accounting for about 90 percent of the dry weight of the mature cell. It consists of heme and globin Understanding the structure of Hemoglobin Hemoglobin molecule is a tetramer consisting of two pairs of polypeptide similar…

How to stain peripheral blood and interpret blood film (Giemsa stain)

How to stain peripheral blood and interpret blood film mltgeeks

A peripheral blood smear or peripheral blood film is a glass slide covered on one side with a thin layer of venous blood. The slide is the stained  and viewed under a microscope.The examination of a stained peripheral blood film is a hematology test and…

Measurement and clinical significance of hematocrit or packed cell volume

HCT PCV TEST

Hematocrit is a frequently performed analysis in haematological laboratories for the evaluation of patients with anaemia.Hematocrit or packed cell volume (HCT / PCV) is defined as the volume occupied by red blood cells after the blood has been centrifuged at high speed. Hematological Methods of…

Blood Transfusion Cross match test (Saline and Indirect Coomb Techniques)

Crossmatch MLTGEEKS

Though used interchangeably, cross matching is only a part of compatibility testing. A full compatibility testing comprises of the following Review of patient’s transfusion history and records An ABO and Rh testing of donor blood group as well as the recipient Antibody screening of the…