Papanicolaou stains : Principle ,Procedure and Interpretation

Papanicolaou stain : Principle ,Procedure and Result Interpretation

Papanicolaou stains are used to differentiate cells in smear preparations of various body secretions. Pap stain is a very reliable technique. As such, it is used for cervical cancer screening.Malignant cells are generally characterized by relatively large nuclei with an abnormal chromatin pattern and small…

Field Stain principle,composition,procedure and interpretation (test to detect malaria)

FIELD STAIN MLTGEEKS

Field’s Stain is a histological staining techniques and romanowsky stain, used for rapid processing of blood specimens and is used to stain thick and thin film (thick film vs thin film) of blood to detect spirochaetes, protozoa and other purposes.It consists of two differential stain.Field…

Schaeffer & Fulton’s Spore Stain principle,procedure and result (endospore stain)

Schaeffer & Fulton's Spore Stain MLTGEEKS

Schaeffer & Fulton’s Spore Stain is a microbiological stain used for staining bacterial spores.A spore is a dormant form of a bacterium that allows it to survive in drastic environmental conditions. Spores have a tough external cover made of protein keratin that is resistant to…

Properties and Principles of Hematoxylin & Eosin staining technique

Hematoxylin and eosin stain mltgeeks

In histopathology,the sections (microtomy) as they are prepared are colourless and different components cannot be appreciated. Staining of tissues(histochemical staining) with different coloured dyeshaving affinities for specific components of the  tissue, makes identification and study of the tissue morphology possible by histotechnologist. There are somany…

Auramine Phenol staining technique for Acid Fast Bacilli

Auramine Phenol stain Mycobacterium tuberculosis MLTGEEKS

Phenolic Auramine is used as primary staining solution in fluorescent staining. It is a staining technique mostly used because of it high sensitivity to detect mycobacterium tuberculosis in sputum,csf and other specimen as compared to ziehl neelsen. It is a fluorochrome technique which enables a…

Lactophenol Cotton Blue stain principle and interpretation for Fungi specimen

LACTOPHENOL COTTON BLUE MTGEEKS

Fungi are members of  the eukaryotic organism and they are classified into two main groups that is yeast and molds.Those fungi capable of exhibiting this to stages are known as dimorphic fungi. Fungi cell wall is made up of chitin and the cells have both…

Gram stain : Principle, preparation of reagents and Interpretation

Gram stain kit MLT GEEKS

Gram stain is  one of the most common staining technique used for examining specimens suspected to contain bacteriologic agents. The direct microscopic examination of specimens and cultures can provide a rapid presumptive diagnosis meanwhile gram staining  is a differential stain that provide morphological information regarding…

Albert staining of metachromatic granules ideal for Corynebacterium diphtheriae

Albert stain MLTGEEKS

This staining technique is used to stain the volutin or metachromatic granules of  corynebacterium diphtheriae,other corynebacterium species and occasionally Bacillus species especially when the organism is cultured on a protein rich medium such as dorset eggs,loeffler serum or Egg yolk agar which makes the volutin…

Acridine Orange staining techniques ideal for Trichomonas vaginalis

Acridine orange film of vaginal swab MLTGEEKS.JPG

This staining technique has been recommended for the rapid identification of Trichomonas vaginalis ,clue cells and yeast cells in genital tract specimens.It is also used to detect intracellular meningococci,gonococci  in cervical and urethral smears and other bacteria such as Helicobacter pylori in gastric biopsies mostly…

Acid Fast Bacilli Ziehl Neelsen staining technique Principle, Procedure and reporting

Avonchem_ZN-TB

Acid-fastness  refers to  a physical property of certain bacterial or group of bacteria and eukaryotic cells, as well as some sub-cellular structures found in a organism, which makes it resistant to decolorization by the acid alcohol during laboratory staining procedures Acid-fast organisms are characterized by…