Mannitol Salt Agar (Selective and different culture media) for Halophilic bacteria

Mannitol salt agar MLTGEEKS

Abbreviate MSA, Mannitol salt agar  is a selective and differential culture medium used in microbiology for the growth and isolation of Gram positive Staphylococci species such as Staphylococcus aureus. It was described by Chapman and has been adopted by many official organisations. Several modifications of it have been developed, all formulations…

Thiosulfate Citrate Bile Sucrose Agar ideal for vibrio species

Abbreviated TCBS, Thiosulfate-Citrate-Bile-Sucrose Agar was original developed by Nakanishi but was subsequently modified by Kobayashy et al. TCBS is frequently used for the selective isolation of Vibrio cholerae and other enteropathogenic like Vibrios parahaemolyticus from suspected specimen. TCBS agar is both selective and differential culture…

CLED Agar Composition, Preparation and Colony growth Characteristics

cled agar MLTGEEKS

Cystine Lactose Electrolyte-Deficient Agar(CLED Agar)  is a type of differential culture media in microbiology recommended for the diagnosis of urinary tract infections. The medium supports the growth of all urinary potential pathogens and provides distinct colony morphology.It also supports the growth of a number of contaminants…

MacConkey Agar Principles, Composition, Preparation, colony characteristics

MacConkey Agar principle and interpretation MLTGEEKS

The culture media MacConkey agar was developed in 20th century by Alfred Theodore MacConkey. MacConkey agar was the first formulated solid differential media but now known as   a selective and differential culture media commonly used for the isolation of enteric Gram-negative bacteria.Based on the…

Sabouraud Dextrose Agar (SDA) Composition, Principle, Uses, Preparation culture and Colony Morphology

Rhodotorula_mucilaginosa_colonies MLTGEEKS

Sabouraud Agar or Sabouraud Dextrose Agar or SDA is a type of agar growth medium containing peptones. SDA  is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as Nocardia. SDA  was created by Raymond Sabouraud in 1892 reason…

Eosin Methylene Blue (EMB) Agar : Principle purpose and colonies characteristics

Eosin Methylene Blue MLTGEEKS

Eosin Methylene Blue (EMB, also known as “levine emb agar formulation”) is a selective and differential culture medium for gram-negative bacteria. It’s contains dyes that are toxic to gram-positive bacteria making it selective. EMB is a selective and differential medium for coliforms bacteria. It is made up of…