Potato Dextrose Agar Principle,Composition and Colony characteristics

Black Mold Aspergillus niger on Potato Dextrose agar

Potato dextrose agar is a fungi growth media recommended for the isolation and counting of yeasts and moulds from dairy products and other foodstuffs. It is also used for stimulating sporulation,maintaining stock cultures of certain dermatophytes and for the differentiation of typical varieties of dermatophytes…

Nitrate broth and Nitrate Reduction Test Principle and Result interpretation

Nitrate broth and Nitrate Reduction test MLTGEEKS

Nitrate Broth is a growth media used to carryout  the biochemical test ”nitrate reductase test”.The purpose of nitrate reduction test is to detect nitrate reduction by some bacteria. The ability to reduce nitrate by the enzyme Nitrate reductase found in some bacteria is valuable for differentiating…

New York City Agar : Principle and Cultural Responses to gonococci species

new yolk city agar MLTGEEKS

New York City Agar abbreviated NYC Agar is an enrichment culture media recommended for the selective isolation of gonococci species such as Neisseria species. It was originally developed by Fauer, Weisburd and Wilson at the New York City Department of Health for selective isolation of…

Thioglycollate Broth principles and Bacteria pattern of Growth

Fluid Thioglycollate medium mltgeeks

Thioglycollate Broth or Fluid Thioglycollate medium is used for sterility testing of antibiotics, biologicals specimen such as stored blood in blood banks,foods and for determination of the phenol coefficient and sporicidal effect of disinfectants.It is also used for the culture and isolation of obligate,facultative anaerobic…

Alkaline Peptone Water an Enrichment Medium for Vibrio Species

alkaline peptone water MLTGEEKS

Alkaline Peptone Water is an enrichment liquid medium which is recommended for the enrichment  of clinical materials containing small numbers of Vibrio species prior to plating onto a selective medium, such as TCBS Agar.It is a modification of peptone water with an  adjusted of pH…

Hippurate Broth Principles and Hippurate Hydrolysis Test

Hippurate Test

Hippurate Broth is a liquid medium which is recommended for use in qualitative determination of the ability of an organism producing the enzyme Hippuricase to hydrolyze sodium hippurate mainly Streptococcal species such as  Streptococcus agalactiae Principle of Hippurate Broth Peptones and heart infusion supply nitrogenous…

Bismuth Sulphite Agar ideal for the isolation or Salmonella species

Salmonella on Bismuth sulfite agar MLTGEEKS

Bismuth Sulphite Agar Medium is a grow medium recommended for the selective isolation of Salmonellae from faeces, urine, sewage and other materials This growth medium is prepared in accordance with USP  and is employed for the isolation and preliminary identification of Salmonella Typhi and other…

PYR Broth Test and Disc method For Steptococcus pyogenes

PYR TEST MLTGEEKS

PYR broth  is generally used for the isolation and identification of Streptococcus pyogenes .It is highly sensitive test and often replaces bacitracin and salt tolerance tests. It is a presumptive test for both group A and D enterococcal streptococci which determine the hydrolysis activity of…

Kligler Iron Agar principles,composition and slants interpretation

Kligler iron slant MLTGEEKS

Kligler Iron Agar abbreviated KIA is a growth media recommended for the differential identification of gram-negative enteric bacilli from clinical and non clinical samples based on the fermentation  sugars that is dextrose, lactose and the production of Hydrogen sulphide Those bacteria that ferment any of…

Brain infusion Broth and Agar Principle and composition and growth response

Brain heart infusion agar MLTGEEKS

Brain heart infusion (BHI) is a nutritious growth medium used to culture a variety of fastidious organisms associated with blood culture work and allied pathological investigations. This nutritious medium has been used to culture pathogenic cocci such as streptococci, pneumococci and meningococci  which are can…