Microbiological culture methods

In Microbiology laboratory, Culture methods are very crucial as it increases the chances of multiplication and identification of microorganism. The following are various reasons for culturing microorganisms in the laboratory: Isolate bacteria in pure culture and identify the…

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium (Oxidative-Fermentative Medium ) is used to distinguish between anaerobic and aerobic breakdown of carbohydrate( glucose).Hugh Leifson Medium was developed by Hugh and Leifson.They described the taxonomic significance of fermentation and oxidative metabolism of carbohydrates in…

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium is used in the isolation and cultivation of Mycobacterium species.Solid media used for the isolation and cultivation of Mycobacteria are egg- based or agar- based.Egg- based media contain whole eggs or egg yolk, potato flour,…

Buffered charcoal yeast extract agar base mltgeeks

How to culture on buffered charcoal yeast extract agar

Buffered charcoal yeast extract agar base (BCYE) with added supplements is used for the selective culture of Legionella species and legionella isolation from clinical and other samples.Legionella species do not form spores and are narrow gram negative rods.Legionella…

Cooked meat medium MLTGEEKS

Robertson’s Cooked Meat (RCM) Medium ideal for the culture of fastidious anaerobic bacteria

Cooked meat medium( rcm media) is a types of culture media which was was originally developed by Robertson  for the cultivation of aerobes and anaerobes in particular pathogenic clostridia species and for the maintenance of stock cultures.The present…

R2a Agar MLTGEEKS

R2A Agar principle and cultural response (ideal for water treatment)

Reasoner’s 2A agar is used for the heterotrophic plate count of treated potable water using longer incubation periods.The heterotrophic plate count (HPC), formerly known as the standard plate count is a procedure for estimating the number of live…

Columbia agar MLTGEEKS

How to prepare Blood and Chocolate agar using Columbia Agar base

Columbia Agar Base is used as an efficient base for the preparation of culture media like blood and chocolate agar .It is also used for the preparation of various selective and differential media and for the isolation of…

Tryptic soy agar MLTGEEKS

How to prepare tryptic Soy Agar(TSA) : Principle,Composition and Colony response

Tryptic Soy Agar plates abbreviated TSA is a general purpose growth medium suitable for the isolation of fastidious and non fastidious microorganisms,culture of both aerobes and anaerobes microorganism.Tryptic Soy Agar slant or Tryptic soy broth (tsb broth) is…

Dermatophyte Test Medium MLTGEEKS

Dermatophyte Test Medium principle and colonies morphology

Dermatophyte Test Medium abbreviated DTM is  a growth selective growth medium used for the selective isolation of dermatophytes. Dermatophytes are a distinct group of fungi that infect the skin,hair and nails of humans and animals engendering a variety…

Black Mold Aspergillus niger on Potato Dextrose agar

Potato Dextrose Agar Principle,Composition and Colony characteristics

Potato dextrose agar is a fungi growth media recommended for the isolation and counting of yeasts and moulds from dairy products and other foodstuffs. It is also used for stimulating sporulation,maintaining stock cultures of certain dermatophytes and for…