Overview of Bacteria identification test procedures and Interpretations

Overview of Bacteria identification test procedures and Interpretations

Isolated bacteria must be further identified at genus and species level. The identification of bacteria is required to cure the disease or infection caused by the bacteria by using appropriate antibiotics. Identification is also important for epidemiological purposes. How to identify bacteria in the laboratory…

10% KOH Test for fungal element identification

Fungal test MLTGEEKS

KOH test is an easy microbiology test used for the identification of fungal elements or skin fungus from a yeast infection, candida infection and hyphae in skin scraping ,nail samples and other biological samples such as  mucous membranes from vaginal infection test , bronchial aspirate or…

Optochin Test – Presumptive Diagnosis Of Streptococcus Pneumoniae

Optochin Test - Presumptive Diagnosis Of Streptococcus pneumoniae MLTGEEKS

Alpha haemolytic streptococci species and Streptococcus pneumoniae cannot be easily distinguished when cultured on Blood Agar plates as pneumococci strain shows partial clearing of blood resulting to a greenish discolouration (alpha-hemolysis).Optochin or ethylhydrocupreine is a chemical substance used in culture media for the differentiation of…

Methyl Red Test Principle,Procedure and Result interpretation

Methyl red test MLTGEEKS

MR Test is a quantitative biochemical tests for identification of bacteria by detection of acid production which requires positive organisms to produce strong acid (lactic, formic) from glucose through the mixed acid fermentation pathway.Methyl red is a pH indicator having a range between 6.0(yellow) and…

Phenylalanine Deamination test procedure and result interpretation

Phenylalanine Deamination test MLTGEEKS

The ability of Proteus species to be able to deaminate phenylalanine to phenylpyruvic acid  using the enzyme Phenylalanine ammonia-lyase (pal enzyme) is an important and useful reaction in the differentiation of members of the family of Enterobacteriaceae.This biochemical tests for identification of bacteria  is used…

Nitrate broth and Nitrate Reduction Test Principle and Result interpretation

Nitrate broth and Nitrate Reduction test MLTGEEKS

Nitrate Broth is a growth media used to carryout  the biochemical test ”nitrate reductase test”.The purpose of nitrate reduction test is to detect nitrate reduction by some bacteria. The ability to reduce nitrate by the enzyme Nitrate reductase found in some bacteria is valuable for differentiating…

Hippurate Broth Principles and Hippurate Hydrolysis Test

Hippurate Test

Hippurate Broth is a liquid medium which is recommended for use in qualitative determination of the ability of an organism producing the enzyme Hippuricase to hydrolyze sodium hippurate mainly Streptococcal species such as  Streptococcus agalactiae Principle of Hippurate Broth Peptones and heart infusion supply nitrogenous…

Candida albican germ tube test procedure and interpretation

Germ tube test MLT GEEKS

Germ tubes are short  outgrowth, non-septate germinating tube-like structures called pseudohyphae produced by Candida albicans when incubated at a temperature of 35-37°C for 2-4 hours in pooled human serum. The use of human serum as a germ tube induction medium has raised questions about standardization,…

PYR Broth Test and Disc method For Steptococcus pyogenes

PYR TEST MLTGEEKS

PYR broth  is generally used for the isolation and identification of Streptococcus pyogenes .It is highly sensitive test and often replaces bacitracin and salt tolerance tests. It is a presumptive test for both group A and D enterococcal streptococci which determine the hydrolysis activity of…

Dnase differential test Principle,Procedure and Result

dnase test MLTGEEKS

Deoxyribonuclease abbreviated DNase  is an enzyme that catalyzes  the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thereby degrading DNA. Some microorganisms like Staphylococcus species possesses this enzymes hence Dnase test agar is a differential medium that test the ability of Dnase producing microorganisms…