Culture methods in Microbiology

In Microbiology laboratory, Culture methods are very crucial as it increases the chances of multiplication and identification of microorganism. The following are various reasons for culturing microorganisms in the laboratory: Isolate bacteria in pure culture and identify the same by performing various tests. In order…

Difference Between Fat-Soluble and Water-Soluble Vitamins

Fruits and their vitamin content

Vitamins can be defined as organic compounds occurring in small quantities in different natural foods and are necessary for growth and maintenance of good health. Vitamins can also be defined as essential food factors, which are required for the proper utilization of food such as…

Cells, Tissues, Organs, Organ Systems

cells tissues organs and systems

Cells, Tissues, Organs, Organ Systems made up the 4 level of organisation of an organism. A cell is the smallest structural and functional unit of a living organism. Bacteria are unicellular consisting of a single cell. Human are multicellular. A group of similar cells having…

Entamoeba coli infection and treatment

Entamoeba coli one small one large cyst iodine

Entamoeba coli can be found worldwide.It is a non pathogenic species of Entamoeba causing Intestinal amebiasis, amebic colitis, amebic dysentery, extraintestinal amebiasis.It’s occurs in cold climates, such as Alaska, as well as warm climates.Geographic areas with poor hygiene and sanitation practices are at the greatest…

Echinococcus granulosus laboratory diagnosis

echinococcus granulosus

Also known as hydatid worm, hyper tapeworm or dog tapeworm, Echinococcus granulosus is primarily found in areas where sheep or other herbivores are raised and are in close contact with dogs or wild canines. Other criteria for at-risk areas include those where there is close…

Loa Loa Filariasis Laboratory diagnosis and treatment

Loa Loa Filariasis (Loiasis) Laboratory diagnosis and treatment

Like all filarial organisms, the endemic regions of infections correlate with the areas in which the vector flourishes.In the case of Loa loa filariasis, Chrysops fly lives in Africa, especially in the rainforest belt region. In the areas in which a large vector population exists,…

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium : Principle, Preparation and Colony Morphology

Hugh Leifson Medium (Oxidative-Fermentative Medium ) is used to distinguish between anaerobic and aerobic breakdown of carbohydrate( glucose).Hugh Leifson Medium was developed by Hugh and Leifson.They described the taxonomic significance of fermentation and oxidative metabolism of carbohydrates in gram- negative intestinal bacteria.There are two ways…

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

Proteus and Providencia Species : Morphology,Diagnosis and Cultural characteristics

The Proteus and Providencia genera belong to the Proteeae of the Enterobacteriaceae family. The members of both these genera are gram negative, motile bacilli, aerobic and facultative anaerobic and can grow in basic media.A characteristic feature that distinguishes tribe Proteeae from other members of Enterobacteriaceae…

Overview of Bacteria identification test procedures and Interpretations

Overview of Bacteria identification test procedures and Interpretations

Isolated bacteria must be further identified at genus and species level. The identification of bacteria is required to cure the disease or infection caused by the bacteria by using appropriate antibiotics. Identification is also important for epidemiological purposes. How to identify bacteria in the laboratory…

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas aeruginosa : Morphology,Diagnosis and Cultural characteristics

Pseudomonas is a bacteria mostly saprophytic in nature, found in soil, water and other moist environments. It has emerged as an important cause of associated health care and opportunistic infections.Most Pseudomonas clinical isolates are resistant to many antibiotics. Pseudomonas aeruginosa is also a plant pathogen….