Acanthamoeba infection laboratory diagnosis

Acanthamoeba infection laboratory diagnosis

Over the years, Acanthamoeba cases have been reported from many countries worldwide. In the United States, both CNS and Acanthamoeba eye infections have been reported. CNS infections occur primarily in patients who are immunocompromised or weakened.Contact lenses wearers, particularly those with soft contacts, may be…

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium : Principle, Preparation and Colony Morphology

Lowenstein-Jensen (LJ) Medium is used in the isolation and cultivation of Mycobacterium species.Solid media used for the isolation and cultivation of Mycobacteria are egg- based or agar- based.Egg- based media contain whole eggs or egg yolk, potato flour, salts and glycerol and are solidified by…

WBC Count : Principle, Procedure and Calculation

WBC (White Blood Cell) Count: Purpose, Procedure, and Results

follow url WBC diluting fluid is used to perform the WBC(leucocyte) count or white blood cell count Principle of WBC Diluting Fluid WBC diluting fluid is used to perform the WBC( leucocyte) count. Red cells are lysed by glacial acetic acid. Gentian violet slightly stains the leukocyte…

Semen Analysis: Sperm Counting Test Procedure and Results

Semen Analysis: Sperm Counting Test Procedure and Results

Semen analysis provides essential information on the individual’s clinical status. Clearly, the collection and analysis of semen must be carried out using properly standardized procedures if the results are to provide valid information.Normal semen is a mixture of sperm suspended in testis secretions and epididymis…

Papanicolaou stains : Principle ,Procedure and Interpretation

Papanicolaou stain : Principle ,Procedure and Result Interpretation

Papanicolaou stains are used to differentiate cells in smear preparations of various body secretions. Pap stain is a very reliable technique. As such, it is used for cervical cancer screening.Malignant cells are generally characterized by relatively large nuclei with an abnormal chromatin pattern and small…

Pneumocystis jiroveci Laboratory Diagnosis

Pneumocystis jiroveci Laboratory Diagnosis

Pneumocystis jiroveci is common in many parts of the world. Particularly in United States, Asia and Europe. The route of organism transmission is believed to be through the transfer of pulmonary droplets by direct person- to- person contact.The population most at risk for Pneumocystis jiroveci…

Minerals Functions,Sources and Deficiency

Minerals : Their Functions,Sources and Deficiency

Minerals are essential for the normal growth and maintenance of the body. If the daily requirement is more than 100 mg / day, they are called major elements and if the daily requirements is less than 100 mg / day, they are called minor elements….

Cryptosporidium parvum laboratory diagnosis

Cryptosporidium parvum laboratory diagnosis

Cryptosporidium is distributed worldwide.Of the 20 species that are known to exist, only Cryptosporidium parvum infects human beings.Infection appears to occur primarily through water or food contaminated with infected feces and through transmission from person to person.Immunocompromised persons are at risk of contracting this parasite,…

Toxoplasma gondii laboratory diagnosis

Toxoplasma gondii laboratory diagnosis

Toxoplasma gondii is found worldwide, mainly because so many animals can harbor the organism.Information collected to date indicates that no population is exempt from the possibility of contracting Toxoplasmosis.One of the most important populations at risk of contracting this parasite are AIDS patients.Several epidemiological considerations…

Wuchereria bancrofti Laboratory Diagnosis

Wuchereria bancrofti Laboratory Diagnosis

Wuchereria bancrofti can be found in the subtropical and tropical areas of the world.These include Central Africa, the Nile Delta, India, Pakistan, Thailand, the Arabian Coast, the Philippines, Japan, Korea and China in the Eastern Hemisphere and Haiti, the Dominican Republic, Costa Rica and the…